All Episodes
Displaying episodes 1 - 30 of 64 in total
5 Principles for Creating a Rewarding Career Inside or Outside of the Lab
#64 — Only a tiny percentage of bioscience PhD students will progress to a PI position. This statistic means that ~95% of us need to find a career path other than the ...

Precise, high-efficiency editing of stem cells to probe human biology and model disease
#63 — Discover how combining two nobel-prize winning technologies—cell reprogramming and CRISPR gene editing—creates a powerful platform to study basic cell biology, d...

Base editing in practice
#62 — Base editing allows researchers to make precise changes to the genome. Discover how this technique works, how it differs from traditional genome editing, and how...

CRISPRi as an alternative to CRISPR-cut in human iPSCs
#61 — Get help and advice with designing and using CRISPRi in human iPSCs. Discover that CRISPRi is an efficient way to silence transcription, sometimes in a complete ...

The 4 D’s of CRISPR gene editing: Design, Develop, Deliver and Detect
#60 — Whether you are just getting started with CRISPR or want to refresh the basics in designing and performing CRISPR experiments, Dr. Antony Adamson can help. [1] A...

The World of Macrophages: How to Harness Them for Research
Although macrophages were first described by Elie Metchnikoff in 1882, plenty of mysteries are still associated with the cell type. Indeed, while macrophages were once...

Basics of FLIM and Applications to FRET Biosensors
Functional imaging is a rapidly growing field key to driving new understanding in biology. Insights into the function and interaction of molecules are the key to revea...

Adding Multiplex Factor to Confocal Imaging with ZEISS LSM
In this tutorial on confocal imaging, you will learn how you can:– Capture weaker signals—and still get sound, reproducible data.– Reach faster volumetric imaging with...

Cryo-electron Tomography for Cell Biology
In this webinar, you will learn:– The complete workflow for in situ cryo-electron tomography– How subtomogram averaging within the cell yields native-state structures ...

Cryo-Electron Microscopy and the Complexity of Cancer
In this webinar, you will learn:– How structural biology can change the face of your research– The biological challenges of cancer research– Advantages of cryo-EM for ...

Fast superresolution microscopy – with Lattice SIM
Your life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working wi...

Array Tomography for SEM 3D Reconstruction
Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and or...

Expanding the limits of EM sample prep with ICE
In this webinar you will learn:– Improved vitrification: Specimen vitrification without synchronisation fluid– Vitrification strategies: Optimized freezing for differe...

Using CRISPR/Cas9 to detect DNA sequences with AFM
Join Dr. Jason Reed as he describes a novel method by which endonuclease-inhibited Cas9 can be employed as a programmable biomarker in high-speed atomic force microsco...

How to Use CRISPR to Accelerate Cancer Therapies
Join Theo Roth as he describes his lab’s novel CRISPR-Cas9 genome-targeting system that does not require viral vectors to modify T cell genomes, but instead focuses on...

Nucleic acids 101: Confirming Their Quality
Join us in this webinar as Dr. Victoria Doronina helps you determine the quality of your nucleic acids. In this webinar you will learn:– How to choose the best method ...

Live Cell Isolation by Laser Microdissection
In this webinar, you will learn:– How to best prepare your specimen for live cell isolation by laser microdissection– How to optimize your laser microdissection workfl...

Reliable quantification of fluorescence images
In this webinar, you will learn:– How to overcome issues with stage drift and crosstalk between channels– How to obtain reliable images and reproducible quantification...

Crash Course: Developing a Fool-Proof ELISA
Join us in this webinar featuring Dr. Omonse Talton who will guide you through developing a “fool proof” enzyme-linked immunosorbent assay (ELISA). In this webinar, yo...

Discovering PARP inhibitor resistance with CRISPR
In this webinar, Dr. Stephen Pettitt explains how he applies genome-wide targeted mutagenesis screens to elucidate the genetic basis of drug resistance. Using mouse an...

14 Ways to Measure Immune Cell Activation
We’ll discuss specific assays in each of these categories, the joys and pitfalls of each assay, and recommendations on how to choose the best method. You will learn ti...

A Guide to Target Gene Validation With Quantitative RT-PCR
While next generation sequencing enables researchers to unveil expression levels of the entire genome, qRT-PCR remains the gold standard for measuring transcript level...

How to Ensure Your Cell-Based Assays Are Reproducible
Join us in this webinar featuring Dr. Vicki Doronina as she takes you through vital components of assay design.In this webinar, you will learn:How to choose between im...

Simultaneous Proteomics and Transcriptomics
Join us in this webinar featuring Dr. Marlon Stoeckius as he explains how you can improve your single-cell RNA-sequencing (scRNA-seq) experiments.In this tutorial, you...

Using qPCR to Validate Epigenetic Enrichment of Pathogen DNA
qPCR is one of the most specific and sensitive tools in molecular biology, allowing the quantification of target DNA molecules present at less than 1 in 106. Next Gene...

Super-Resolved STED Spectroscopy: New insights
Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are ...

Challenges and Solutions in Microbiome Analysis Workflows
Elucidating meaningful, unbiased microbial community profiles from complex microbiome samples is challenging. In this webinar, you will learn:– the sources of bias thr...

Precise and Fast Spectral FLIM at Video Rate in Confocal
Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET ...

Complete and Fast 3D Image Analysis in Microscopy
If image analysis is a place you fear to tread, or if you struggle with over complicated and time-consuming microscopy image analysis workflows, this is your opportuni...

A Guide to Harnessing DNA Assembly for Drug Discovery
Current DNA synthesis and assembly technologies give today’s genetic engineers unprecedented freedom to control every aspect of genetic design. In this webinar on DNA ...
