Guide to CRISPR/Cas9 Delivery How to Maximize Editing

In this webinar, you will learn how to maximize your genome editing efficiency using CRISPR/Cas9 and how to apply this technique in your research.

The main points in the webinar will include:

How to design guide RNAs using online tools specific to the genome and application of interest.
Tips and practical advice to assist you in choosing and optimizing a CRISPR/Cas9 delivery system.
How you can create precise mutations using homology-directed repair, including template design and cleavage site
An Essential Guide to CRISPR/Cas9 Editing Efficiency
While CRISPR/Cas9 editing is utilized in a wide variety of cell types, editing efficiency continues to pose a challenge to researchers. Join Dr. Allison Mayle, as she shares best practices for increasing CRISPR/Cas9 editing efficiency. Viewers will discover online tools to aid in CRISPR/Cas9 design and delivery and tips for optimizing your CRISPR/Cas9 experiments.

In this webinar, Dr. Mayle will review the factors influencing genome editing, including target sequence selection and CRISPR delivery methods. A comparison of plasmid and viral vector delivery will be provided, as well as an introduction to DNA-free CRISPR/Cas9 ribonucleoprotein reagents. Additionally, Dr. Mayle will cover best practices for CRISPR knock-in mutagenesis via homology-directed repair (HDR) and applications available from new Cas9 protein variants.

Creators and Guests

Karen O'Hanlon Cohrt
Host
Karen O'Hanlon Cohrt
Editor in Chief, CRISPR Medicine News
Allison Mayle
Guest
Allison Mayle
Science Educator, Assistant Director DNALC NYC
Guide to CRISPR/Cas9 Delivery How to Maximize Editing